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ecl blocking agent in pbst  (Cytiva Europe)


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    Structured Review

    Cytiva Europe ecl blocking agent in pbst
    Ecl Blocking Agent In Pbst, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 94/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/blocking+agent/Amersham+ECL+blocking+agent/pmc12887003-255-17-22
    Average 94 stars, based on 39 article reviews
    ecl blocking agent in pbst - by Bioz Stars, 2026-11
    94/100 stars

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    Related Articles

    Blocking Assay:

    Article Title: Myotube hypertrophy is associated with cancer-like metabolic reprogramming and limited by PHGDH
    Article Snippet: Samples were then electrophoresed on 12% Bis-Tris gels (Bio-rad, Cat#3450125, Hemel Hempstead, UK) and transferred onto PVDF membranes (GE Healthcare, Cat#15269894, Chicago, IL, USA) using a semi-dry transfer blotter (Bio-rad). .. Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389; 1:2000; Cell Signaling Technology, Cat# 9234, Leiden, The Netherlands, RRID:AB_2269803), Phospho-AKT Ser473 (1:2000; Cell Signaling Technology, Cat# 4060, Leiden, The Netherlands, RRID:AB_2315049), α-TUBULIN (1:10000; Cell Signaling Technology, Cat# 2125, Leiden, The Netherlands, RRID:AB_2619646), pan-ACTIN (1:1000, Cell Signaling Technology, Cat# 8456, Leiden, The Netherlands, RRID:AB_10998774), PHGDH (1:1000; Cell Signaling Technology, Cat# 13428, Leiden, The Netherlands, RRID:AB_2750870), Phospho-AMPK (Thr172, 1:500, Cell Signaling Technology, Cat# 2531, Leiden, The Netherlands, RRID:AB_330330), and anti-rabbit/mouse IgG secondary antibody (1:2000; Roche, Cat#12015218001, Basal, Switzerland) prior to fluorescent imaging. .. Densities of the bands from blot images were normalized loading control by densitometric analysis using ImageJ software (RRID:SCR_003070).

    Incubation:

    Article Title: Myotube hypertrophy is associated with cancer-like metabolic reprogramming and limited by PHGDH
    Article Snippet: Samples were then electrophoresed on 12% Bis-Tris gels (Bio-rad, Cat#3450125, Hemel Hempstead, UK) and transferred onto PVDF membranes (GE Healthcare, Cat#15269894, Chicago, IL, USA) using a semi-dry transfer blotter (Bio-rad). .. Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389; 1:2000; Cell Signaling Technology, Cat# 9234, Leiden, The Netherlands, RRID:AB_2269803), Phospho-AKT Ser473 (1:2000; Cell Signaling Technology, Cat# 4060, Leiden, The Netherlands, RRID:AB_2315049), α-TUBULIN (1:10000; Cell Signaling Technology, Cat# 2125, Leiden, The Netherlands, RRID:AB_2619646), pan-ACTIN (1:1000, Cell Signaling Technology, Cat# 8456, Leiden, The Netherlands, RRID:AB_10998774), PHGDH (1:1000; Cell Signaling Technology, Cat# 13428, Leiden, The Netherlands, RRID:AB_2750870), Phospho-AMPK (Thr172, 1:500, Cell Signaling Technology, Cat# 2531, Leiden, The Netherlands, RRID:AB_330330), and anti-rabbit/mouse IgG secondary antibody (1:2000; Roche, Cat#12015218001, Basal, Switzerland) prior to fluorescent imaging. .. Densities of the bands from blot images were normalized loading control by densitometric analysis using ImageJ software (RRID:SCR_003070).

    Imaging:

    Article Title: Myotube hypertrophy is associated with cancer-like metabolic reprogramming and limited by PHGDH
    Article Snippet: Samples were then electrophoresed on 12% Bis-Tris gels (Bio-rad, Cat#3450125, Hemel Hempstead, UK) and transferred onto PVDF membranes (GE Healthcare, Cat#15269894, Chicago, IL, USA) using a semi-dry transfer blotter (Bio-rad). .. Membranes were blocked in prime blocking agent (GE Healthcare, Cat#RPN418, Chicago, IL, USA), then incubated with primary Phospho-P70S6K (Thr389; 1:2000; Cell Signaling Technology, Cat# 9234, Leiden, The Netherlands, RRID:AB_2269803), Phospho-AKT Ser473 (1:2000; Cell Signaling Technology, Cat# 4060, Leiden, The Netherlands, RRID:AB_2315049), α-TUBULIN (1:10000; Cell Signaling Technology, Cat# 2125, Leiden, The Netherlands, RRID:AB_2619646), pan-ACTIN (1:1000, Cell Signaling Technology, Cat# 8456, Leiden, The Netherlands, RRID:AB_10998774), PHGDH (1:1000; Cell Signaling Technology, Cat# 13428, Leiden, The Netherlands, RRID:AB_2750870), Phospho-AMPK (Thr172, 1:500, Cell Signaling Technology, Cat# 2531, Leiden, The Netherlands, RRID:AB_330330), and anti-rabbit/mouse IgG secondary antibody (1:2000; Roche, Cat#12015218001, Basal, Switzerland) prior to fluorescent imaging. .. Densities of the bands from blot images were normalized loading control by densitometric analysis using ImageJ software (RRID:SCR_003070).



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